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KMID : 0617219950060010022
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Duksung Bulletin Phamaceutical Sciences
1995 Volume.6 No. 1 p.22 ~ p.26
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Effect of anesthetics on the hepatic glucuronidation in rats
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Abstract
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As an approach to elucidate the possible involvement of licorice on the glucuronidation in rats, the enzymatic activity of hepatic UDP-glucuronosyltransferase 2 (UDP-GT2) and intracellular concentrations of hepatic UDP-glucuronic acid (UDP-GA) upon the pretreatment of licorice were measured. Anesthetics such as ether and pentobarbital are often administered to the animals before the livers are taken. Ether inhalation has been reported to decrease dramatically the hepatic concentration of UDP-GA when the livers were removed 1 hr after the administration of ether. It is also possible that pentobarbital, a kind of barbiturates, has some effect on the glucuronidation since phenobarbital has been known to affect the enzymatic activity of UDP-GT. In the present study, effect of anesthetics was determined. Enzymatic activity of UDP-GT2 and intracellular concentrations of hepatic UDP-GA upon the pretreatment of Glycyrrhizae Radix(GR, 1g/㎏ body weight, p.o., 6 days) were measured when the livers were removed right after the animals were anesthetized with ether inhalation or pentabarbital treatment (50㎎/㎏. i.p.). GR caused significant increases in specific activities of UCP-GT2 by 79-120% and hepatic concentrations of UDP-GA by 132-241%. Neither ether inhalation nor pentobarbital treatment reduced hepatic UDP-GA concentrations or substantially altered hepatic UDP-GT2 activities. This result demonstrates that the use of ether or pentobarbital did not infuence hepatic glucuronidation when the livers were removed shortly after the anesthetization.
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